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National Research Council, «Putting Biotechnology to Work: Bioprocess Engineering»ISBN: 0309047854 | Publisher: National Academies Press | Publication Date: 1992-11 | Number Of Pages: 132 | pdf | 1,6 mb

Biotechnology is broadly defined in a 1991 Office of Technology Assessment report as "any technique that uses living organisms (or parts of organisms) to make or modify products, to improve plants or animals, or to develop microorganisms for specific uses." This technology has been instrumental in the development and implementation of processes for the manufacture of antibiotics and other pharmaceuticals, industrial sugars, alcohols,amino acids and other organic acids, foods, and specialty products through the application of microbiology, fermentation, enzymes, and separation technology.Engineers, working with life scientists, often achieved scale-up to industrial production in remarkably short periods. A relatively small number helped to catalyze, over a period of 50 years, the growth of the pharmaceutical, food, agricultural-processing, and specialty-product sectors of the U.S. economy to the
point where sales now exceed $100 billion/year.
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Far from being a new branch of scientific discovery, biotechnology has been practiced for thousands of years; it affects everyday life in continuously evolving ways. Biotechnology: Changing Life Through Science aims to explore this complex subject in a way that is understandable for students with an emphasis on real-world applications of biotechnology.

It will explore the relationship between science (the natural world) and technology (the human-made world) and highlight its impact. Entries are separated into three volumes, each with a focus on a specific area of biotechnology impact:

* Volume 1: Medicine (covers health applications)
* Volume 2: Agriculture (covers food, nature and the environment)
* Volume 3: Industry (covers industrial uses)
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Food Biotechnology, Second Edition
CRC Press | ISBN: 0824753291 | 2005-10-11 | PDF | 2008 pages | 29.2 Mb | RS

Immobilization Of Enzymes And Cells (Methods in Biotechnology)
Humana Press | 449 pages | 2006-04-13 | ISBN: 1588292908 | PDF | 3.51MB
The second edition of Immobilization of Enzymes and Cells serves as an update as well as a compliment to the first edition. This volume now includes simple protocols for the immobilization of enzymes and cells that could be useful for application at industrial scale, novel protocols for immobilization in the future, and new chemical reactors able to overcome the limitations of a number of immobilized derivatives.
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Mohamed A. Desai and a team of experienced biotechnologists review both conventional and novel isolation techniques used in industrial applications for the downstream processing of protein molecules. These techniques include primary and secondary separations during the isolation of biomolecules, as well as unique laboratory-scale research methods from academia with a potential for scale-up. Also treated are various strands of the downstream biological process essential for a successful product license application, including both the validation of DSP stages, and the design and validation of viral clearance stages during the purification process. Downstream Processing of Proteins: Methods and Protocols provides scientists everywhere, but particularly in the biopharmaceutical and biotechnology industry, with a much-needed introduction to this critical technology
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This is the first book that provides a comprehensive review of the entire area of artificial cells. The author, a pioneer of the field, invented the first artificial cells some 50 years ago and has continued to carry out active research in this field. Since then, there have been explosive research activities around the world on artificial cells, especially in fields related to biotechnology, nanomedicine, cell therapy, blood substitutes, drug delivery and others. However, instead of the term artificial cells, many authors use other terminologies such blood substitutes, bioencapsulation, liposomes, nanoparticles, and so on. As a result, any meaningful literature search for a complete idea of the present status of the whole field of artificial cells is impossible. Furthermore, the fact that papers in this highly interdisciplinary area are published in numerous journals specializing in chemistry, medicine, surgery, bioengineering, and others, makes literature search even more difficult. Books in this area are mostly multi-authored, describing very specific and narrow areas. Therefore, many who enter this field for the first time cannot find any book that describes laboratory methods and procedures needed for starting their research. This monograph is written to fill this gap. Based on his 50 years of work and an interdisciplinary background, the author has managed to include all those areas in artificial cells that are disguised under different terminologies.
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From the laboratory to full-scale commercial production, this reference provides a clear and in-depth analysis of bioreactor design and operation and encompasses critical aspects of the biocatalytic manufacturing process. It clarifies principles in reaction and biochemical engineering, synthetic and biotransformation chemistry, and biocell and enzyme kinetics for successful applications of biocatalysis and bioprocess technologies in the food, chiral drug, vitamin, pharmaceutical, and animal feed industries. Studying reactions from small to supramolecules, this reference provides an abundant display of end-of-chapter problems to sharpen understanding of key concepts presented in the text.

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Description: Flow cytometry is a sensitive and quantitative platform for the measurement of particle fluorescence. In flow cytometry, the particles in a sample flow in single file through a focused laser beam at rates of hundreds to thousands of particles per second. During the time each particle is in the laser beam, on the order of ten microseconds, one or more fluorescent dyes associated with that particle are excited. The fluorescence emitted from each particle is collected through a microscope objective, spectrally filtered, and detected with photomultiplier tubes. Flow cytometry is uniquely capable of the precise and quantitative molecular analysis of genomic sequence information, interactions between purified biomolecules and cellular function. Combined with automated sample handling for increased sample throughput, these features make flow cytometry a versatile platform with applications at many stages of drug discovery. Traditionally, the particles studied are cells, especially blood cells; flow cytometry is used extensively in immunology. This volume shows how flow cytometry is integrated into modern biotechnology, dealing with issues of throughput, content, sensitivity, and high throughput informatics with applications in genomics, proteomics and protein-protein interactions, drug discovery, vaccine development, plant and reproductive biology, pharmacology and toxicology, cell-cell interactions and protein engineering.

Many scientists find themselves working in the laboratory without sufficient background in current biotechnology methods. Others want to keep up with the revolution in biotechnology and the flood of new methodologies. This book provides a solution for both: a multidisciplinary approach to the methods essential to biotechnical development. Covering state-of-the-art technologies and a broad range of practical applications, the Second Edition of Gene Biotechnology presents information that researchers and students need to understand and apply today's biotechnology techniques. It discusses a wide variety of approaches, from very basic methods to the latest, most sophisticated technologies, including gene manipulation methods. The book contains clearly detailed, step-by-step protocols with helpful troubleshooting tips. It addresses the needs of researchers in academic and commercial environments, and serves as a guide for graduate students designing, implementing, and evaluating experimental projects. Written by a team of internationally recognized scientists, this edition offers several notable features typically lacking in other works on the subject. The book presents protocols, as well as clear and simple explanations of the key principles and concepts behind the methods. It includes useful troubleshooting guides to help solve experimental problems, presenting a single, logically organized source for all the most important new methodologies. This unique resource provides the tools for success in molecular and cellular biology research today.
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Description: This is the first book that provides a comprehensive review of the entire area of artificial cells. The author, a pioneer of the field, invented the first artificial cells some 50 years ago and has continued to carry out active research in this field. Since then, there have been explosive research activities around the world on artificial cells, especially in fields related to biotechnology, nanomedicine, cell therapy, blood substitutes, drug delivery and others. However, instead of the term artificial cells, many authors use other terminologies such blood substitutes, bioencapsulation, liposomes, nanoparticles, and so on. As a result, any meaningful literature search for a complete idea of the present status of the whole field of artificial cells is impossible. Furthermore, the fact that papers in this highly interdisciplinary area are published in numerous journals specializing in chemistry, medicine, surgery, bioengineering, and others, makes literature search even more difficult. Books in this area are mostly multi-authored, describing very specific and narrow areas. Therefore, many who enter this field for the first time cannot find any book that describes laboratory methods and procedures needed for starting their research. This monograph is written to fill this gap. Based on his 50 years of work and an interdisciplinary background, the author has managed to include all those areas in artificial cells that are disguised under different terminologies. Since this is a very large area, he starts each chapter by giving a detailed overview and has included more than 1000 references to facilitate easier literature search of this highly interdisciplinary area. Detailed examples of the author s own research are given and methods and procedures are described in detail. Readers interested in a detailed overview of the whole area can read from cover to cover but omitting the methods section at the end of each chapter. On the other hand, those entering this area of research will find the detailed methods and procedures very useful.

In this updated second edition, leading researchers apply molecular diagnostics to the many recent advances that have occurred in polymerase chain reaction( PCR)-based technologies. Highlights include real-time PCR, which allows the technique to be performed in a quantitative manner with improved sensitivity, robustness, and resilience to carryover contamination, mass spectrometric analysis of nucleic acids, and circulating cell-free nucleic acids in plasma. The authors apply these innovations to a broad spectrum of applications, including gene expression, methylation, trace molecule, gene dosage, and single cell analysis.
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